Anti-Acetyl-Histone H3-K27 Antibody (CAB7253)
- SKU:
- CAB7253
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Application:
- IP
- Application:
- ChIP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Reactivity:
- Other (Wide Range)
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-Acetyl-Histone H3-K27 Antibody |
| 抗体コード: | CAB7253 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF IP ChIP ChIPseq |
| 反応性: | Human, Mouse, Rat, Other (Wide Range) |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic acetylated peptide corresponding to residues surrounding K27 of human Histone H3 |
| 申し込み: | WB IHC IF IP ChIP ChIPseq |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 ChIPseq 1:20 - 1:100 |
| 反応性: | Human, Mouse, Rat, Other (Wide Range) |
| ポジティブサンプル: | BT-474, 293T |
| 免疫原: | A synthetic acetylated peptide corresponding to residues surrounding K27 of human Histone H3 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 8290 |
| Uniprot: | P68431 |
| セルラーロケーション: | Chromosome, Nucleus |
| 計算された分子量: | 15kDa |
| 観察された分子量: | 16kDa |
| 同義語: | H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A |
| バックグラウンド: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| UniProt Protein Function: | H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.2 Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex Molecular Function:cadherin binding; histone binding; protein binding Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere organization and biogenesis |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P68431 |
| NCBI GenInfo Identifier: | 55977055 |
| NCBI Gene ID: | 8357 |
| NCBI Accession: | P68431.2 |
| UniProt Secondary Accession: | P68431,P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9 Q6NXU4, Q71DJ3, Q93081, A0PJT7, A5PLR1, |
| UniProt Related Accession: | P68431 |
| Molecular Weight: | 15kDa |
| NCBI Full Name: | Histone H3.1 |
| NCBI Synonym Full Names: | histone cluster 1 H3 family member h |
| NCBI Official Symbol: | HIST1H3H |
| NCBI Official Synonym Symbols: | H3/k; H3FK; H3F1K |
| NCBI Protein Information: | histone H3.1 |
| UniProt Protein Name: | Histone H3.1 |
| UniProt Synonym Protein Names: | Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l |
| UniProt Gene Name: | HIST1H3A |
 | Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 antibody (CAB7253) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human breast using Acetyl-Histone H3-K27 antibody (CAB7253) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using Acetyl-Histone H3-K27 antibody (CAB7253) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K27 antibody (CAB7253) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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