Anti-AADAT Antibody (CAB13090)
- SKU:
- CAB13090
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-AADAT Antibody |
| 抗体コード: | CAB13090 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 34-429 of human AADAT (NP_001273611.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse liver, Rat kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 34-429 of human AADAT (NP_001273611.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | PKSM ISLA GGLP NPNM FPFK TAVI TVEN GKTI QFGE EMMK RALQ YSPS AGIP ELLS WLKQ LQIK LHNP PTIH YPPS QGQM DLCV TSGS QQGL CKVF EMII NPGD NVLL DEPA YSGT LQSL HPLG CNII NVAS DESG IVPD SLRD ILSR WKPE DAKN PQKN TPKF LYTV PNGN NPTG NSLT SERK KEIY ELAR KYDF LIIE DDPY YFLQ FNKF RVPT FLSM DVDG RVIR ADSF SKII SSGL RIGF LTGP KPLI ERVI LHIQ VSTL HPST FNQL MISQ LLHE WGEE GFMA HVDR VIDF YSNQ KDAI LAAA DKWL TGLA EWHV PAAG MFLW IKVK GIND VKEL IEEK AVKM GVLM LPGN AFYV DSSA PSPY LRAS FSSA SPEQ MDVA FQVL AQLI KESL |
| 遺伝子ID: | 51166 |
| Uniprot: | Q8N5Z0 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 47kDa |
| 観察された分子量: | 47kDa |
| 同義語: | AADAT, KAT2, KATII, KYAT2 |
| バックグラウンド: | This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. |
| UniProt Protein Function: | AADAT: Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino- group acceptors, with a preference for 2-oxoglutarate, 2- oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro). Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.6.1.7; Amino Acid Metabolism - tryptophan; EC 2.6.1.39; Amino Acid Metabolism - lysine degradation; Transferase; Amino Acid Metabolism - lysine biosynthesis; Mitochondrial Chromosomal Location of Human Ortholog: 4q33 Cellular Component: mitochondrial matrix Molecular Function:protein homodimerization activity; kynurenine-oxoglutarate transaminase activity; 2-aminoadipate transaminase activity; pyridoxal phosphate binding Biological Process: glutamate metabolic process; tryptophan catabolic process to kynurenine; L-lysine catabolic process to acetyl-CoA via saccharopine; lysine catabolic process; biosynthetic process; tryptophan catabolic process; 2-oxoglutarate metabolic process |
| NCBI Summary: | This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] |
| UniProt Code: | Q8N5Z0 |
| NCBI GenInfo Identifier: | 33469970 |
| NCBI Gene ID: | 51166 |
| NCBI Accession: | NP_872603.1 |
| UniProt Secondary Accession: | Q8N5Z0,Q9UL02, B3KP84, |
| UniProt Related Accession: | Q8N5Z0 |
| Molecular Weight: | 47,822 Da |
| NCBI Full Name: | kynurenine/alpha-aminoadipate aminotransferase, mitochondrial isoform b |
| NCBI Synonym Full Names: | aminoadipate aminotransferase |
| NCBI Official Symbol: | AADAT |
| NCBI Official Synonym Symbols: | KAT2; KATII; KYAT2 |
| NCBI Protein Information: | kynurenine/alpha-aminoadipate aminotransferase, mitochondrial |
| UniProt Protein Name: | Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial |
| UniProt Synonym Protein Names: | 2-aminoadipate aminotransferase; 2-aminoadipate transaminase (EC:2.6.1.39); Alpha-aminoadipate aminotransferase; AadAT; Kynurenine aminotransferase II; Kynurenine--oxoglutarate aminotransferase II; Kynurenine--oxoglutarate transaminase 2 (EC:2.6.1.7); Kynurenine--oxoglutarate transaminase II |
| Protein Family: | Kynurenine/alpha-aminoadipate aminotransferase |
| UniProt Gene Name: | AADAT |
| UniProt Entry Name: | AADAT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AADAT antibody (CAB13090) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver using AADAT Rabbit pAb (CAB13090) at dilution of 1:200 (40x lens). |
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