AMPK (G1/B2/A2) Recombinant Protein (RPES2416)
- SKU:
- RPES2416
- Product type:
- Recombinant Protein
- Host species:
- Baculovirus-Insect Cells
- Reactivity:
- Human
Frequently bought together:
Description
system_update_altデータセット
製品名: td> | AMPK (G1/B2/A2) Recombinant Protein (RPES2416) |
製品コード: td> | RPES2416 |
サイズ: td> | 20µg |
由来種: | Human |
発現宿主: | Baculovirus-Insect Cells |
同義語: td> | AMPK,AMPKa1 |
アクセッション: td> | P54619 |
タンパク質配列: | Met 1-Pro 331 |
フュージョンタグ: | |
アクティビティ: | The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate. |
エンドトキシン: | <1.0 EU per µg of the protein as determined by the LAL method. |
タンパク質の構築: | A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331); constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272); constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus; constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. |
純度: | > 90 % as determined by reducing SDS-PAGE. |
分子量: | 158 (38+30+90) kDa |
見かけの分子量: | 35; 37 & 95 kDa |
構成: | Supplied as sterile 50mM Tris; 200mM NaCl; 1mM EDTA; 1mM DTT; 0.5mM PMSF; 10% gly; 1mM GSH; pH 7.4 |
運送: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C. |
安定性と保管: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |