AMPK (G1/B1/A2) Recombinant Protein (RPES0595)
- SKU:
- RPES0595
- Product type:
- Recombinant Protein
- Host species:
- Baculovirus-Insect Cells
- Reactivity:
- Human
Frequently bought together:
Description
system_update_altデータセット
| 製品名: | AMPK (G1/B1/A2) Recombinant Protein (RPES0595) |
| 製品コード: | RPES0595 |
| サイズ: | 20µg |
| 由来種: | Human |
| 発現宿主: | Baculovirus-Insect Cells |
| 同義語: | AMPK,AMPKa1 |
| アクセッション: | P54619 |
| タンパク質配列: | Met 1-Pro 331 |
| フュージョンタグ: | |
| アクティビティ: | The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate. |
| エンドトキシン: | <1.0 EU per µg of the protein as determined by the LAL method. |
| タンパク質の構築: | A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331); constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270); constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus; constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. |
| 純度: | > 94 % as determined by reducing SDS-PAGE. |
| 分子量: | 158 (38+30+90) kDa |
| 見かけの分子量: | 40; 42 & 95 kDa |
| 構成: | Supplied as sterile 50mM Tris; 200mM NaCl; 1mM EDTA; 1mM DTT; 0.5mM PMSF; 10% gly; 1mM GSH; pH 7.4 |
| 運送: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C. |
| 安定性と保管: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
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