AGXT2 Antibody (PACO57460)
- SKU:
- PACO57460
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | AGXT2 Antibody (PACO57460) |
抗体コード: | PACO57460 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
反応性: | Human |
免疫原: | Recombinant Human Alanine--glyoxylate aminotransferase 2, mitochondrial protein (196-328AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: LO2 whole cell lysate. All lanes: AGXT2 antibody at 4.9µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 50 kDa. Observed band size: 50 kDa. | |
IHC image of PACO57460 diluted at 1:700 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of HepG2 cells with PACO57460 at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Can metabolize asymmetric dimethylarginine (ADMA) via transamination to alpha-keto-delta-(NN-dimethylguanidino) valeric acid, (DMGV). ADMA is a potent inhibitor of nitric-oxide (NO) synthase, and this activity provides mechanism through which the kidney regulates blood pressure. |
シノニム: | Alanine--glyoxylate aminotransferase 2, mitochondrial (AGT 2) (EC 2.6.1.44) ((R)-3-amino-2-methylpropionate--pyruvate transaminase) (EC 2.6.1.40) (Beta-ALAAT II) (Beta-alanine-pyruvate aminotransferase) (D-AIBAT), AGXT2, AGT2 |
UniProt Protein Function: | AGXT2: is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008] |
UniProt Protein Details: | Protein type:EC 2.6.1.40; Mitochondrial; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.44; Transferase Chromosomal Location of Human Ortholog: 5p13 Cellular Component: mitochondrion; mitochondrial matrix Molecular Function:beta-alanine-pyruvate transaminase activity; alanine-glyoxylate transaminase activity; (R)-3-amino-2-methylpropionate-pyruvate transaminase activity; pyridoxal phosphate binding Biological Process: pyrimidine nucleoside catabolic process; positive regulation of nitric oxide biosynthetic process; glyoxylate catabolic process; pyrimidine base metabolic process; glyoxylate metabolic process; nucleobase, nucleoside and nucleotide metabolic process; glycine biosynthetic process, by transamination of glyoxylate; L-alanine catabolic process, by transamination |
NCBI Summary: | The protein encoded by this gene is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008] |
UniProt Code: | Q9BYV1 |
NCBI GenInfo Identifier: | 17432913 |
NCBI Gene ID: | 64902 |
NCBI Accession: | Q9BYV1.1 |
UniProt Secondary Accession: | Q9BYV1,Q53FB4, Q53FY7, Q53G03, Q5W7Q1, B7ZM47, E9PDL7 |
UniProt Related Accession: | Q9BYV1 |
Molecular Weight: | 49,297 Da |
NCBI Full Name: | Alanine--glyoxylate aminotransferase 2, mitochondrial |
NCBI Synonym Full Names: | alanine--glyoxylate aminotransferase 2 |
NCBI Official Symbol: | AGXT2 |
NCBI Official Synonym Symbols: | AGT2; DAIBAT |
NCBI Protein Information: | alanine--glyoxylate aminotransferase 2, mitochondrial; beta-ALAAT II; beta-alanine-pyruvate aminotransferase; (R)-3-amino-2-methylpropionate--pyruvate transaminase |
UniProt Protein Name: | Alanine--glyoxylate aminotransferase 2, mitochondrial |
UniProt Synonym Protein Names: | (R)-3-amino-2-methylpropionate--pyruvate transaminase (EC:2.6.1.40); Beta-ALAAT II; Beta-alanine-pyruvate aminotransferase; D-AIBAT |
UniProt Gene Name: | AGXT2 |
UniProt Entry Name: | AGT2_HUMAN |
Antibodies |
Anti-AGXT2 Antibody (CAB18486) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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