ADH1A Antibody (PACO55382)
- SKU:
- PACO55382
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | ADH1A Antibody (PACO55382) |
抗体コード: | PACO55382 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Alcohol dehydrogenase 1A protein (104-253AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO55382 diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of HepG2 cells with PACO55382 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO55382 diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | cytosol, nucleoplasm, plasma membrane, alcohol dehydrogenase (NAD) activity, alcohol dehydrogenase activity, zinc-dependent, retinol dehydrogenase activity, alcohol metabolic process, drug metabolic process, ethanol oxidation |
シノニム: | Alcohol dehydrogenase 1A (EC 1.1.1.1) (Alcohol dehydrogenase subunit alpha), ADH1A, ADH1 |
UniProt Code: | P07327 |
NCBI GenInfo Identifier: | 4501929 |
NCBI Gene ID: | 124 |
NCBI Accession: | NP_000658.1 |
UniProt Secondary Accession: | P07327,Q17R68, A8K3E3, |
UniProt Related Accession: | P07327 |
Molecular Weight: | 375 |
NCBI Full Name: | alcohol dehydrogenase 1A |
NCBI Synonym Full Names: | alcohol dehydrogenase 1A (class I), alpha polypeptide |
NCBI Official Symbol: | ADH1A |
NCBI Official Synonym Symbols: | ADH1 |
NCBI Protein Information: | alcohol dehydrogenase 1A; ADH, alpha subunit; aldehyde reductase; alcohol dehydrogenase subunit alpha; alcohol dehydrogenase 1 (class I), alpha polypeptide |
UniProt Protein Name: | Alcohol dehydrogenase 1A |
UniProt Synonym Protein Names: | Alcohol dehydrogenase subunit alpha |
Protein Family: | Alcohol dehydrogenase |
UniProt Gene Name: | ADH1A |
UniProt Entry Name: | ADH1A_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |