ADD1 (Ab-726) Antibody (PACO23564)
- SKU:
- PACO23564
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | ADD1 (Ab-726) Antibody (PACO23564) |
抗体コード: | PACO23564 |
サイズ: | 100ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
反応性: | Human, Mouse, Rat |
免疫原: | Peptide sequence around aa.724~728 (T-P-S-F-L) derived from Human ADD1. |
憲法: | Liquid |
ストレージバッファ: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from 293 and Hela cells using ADD1(Ab-726) Antibody. | |
Immunofluorescence staining of methanol-fixed Hela cells using ADD1(Ab-726) Antibody. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1(Ab-726) Antibody(left) or the same antibody preincubated with blocking peptide(right). |
バックグラウンド: | Adducins are a family of cytoskeleton proteins encoded by three genes (a, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. a- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. a- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. |
シノニム: | ADDA; Erythrocyte adducin alpha subunit; |
UniProt Protein Function: | Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. |
NCBI Summary: | Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017] |
UniProt Code: | P35611 |
NCBI GenInfo Identifier: | 12644231 |
NCBI Gene ID: | 118 |
NCBI Accession: | P35611.2 |
UniProt Secondary Accession: | P35611,Q13734, Q14729, Q16156, Q86XM2, Q9UJB6, A2A3N8 A2A3P0, B4DI79, D3DVR3, D3DVR4, D3DVR5, |
UniProt Related Accession: | P35611 |
Molecular Weight: | 73,333 Da |
NCBI Full Name: | Alpha-adducin |
NCBI Synonym Full Names: | adducin 1 |
NCBI Official Symbol: | ADD1 |
NCBI Official Synonym Symbols: | ADDA |
NCBI Protein Information: | alpha-adducin |
UniProt Protein Name: | Alpha-adducin |
UniProt Synonym Protein Names: | Erythrocyte adducin subunit alpha |
Protein Family: | Alpha-adducin |
UniProt Gene Name: | ADD1 |
Antibodies |
Anti-ADD1 Antibody (CAB1592) |
Anti-Phospho-Alpha-adducin-S726 Antibody (CABP0196) |
Phospho-ADD1 (T445) Antibody (PACO03410) |
ADD1 Antibody (PACO03411) |
ADD1 Antibody (PACO07612) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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