ABCB4 Antibody (PACO54602)
- SKU:
- PACO54602
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | ABCB4 Antibody (PACO54602) |
抗体コード: | PACO54602 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:200-1:500 |
反応性: | Human |
免疫原: | Recombinant Human Phosphatidylcholine translocator ABCB4 protein (530-693AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | Immunohistochemistry of paraffin-embedded human melanoma using PACO54602 at dilution of 1:100. |
![]() | Immunofluorescence staining of A549 cells with PACO54602 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts. Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to fu rther enrichment of SM and cholesterol in raft membranes in hepatocytes. Required for proper phospholipid bile formation (By similarity). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner. May promote biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane. In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts. Does not confer multidrug resistance (By similarity). |
シノニム: | Phosphatidylcholine translocator ABCB4 (ATP-binding cassette sub-family B member 4) (Multidrug resistance protein 3) (EC 3.6.3.44) (P-glycoprotein 3), ABCB4, MDR3 PGY3 |
UniProt Protein Function: | ABCB4: a transporter that mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human ABCB4 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. Interacts with HAX1. Defects are the cause of gallstones, and progressive familial intrahepatic cholestasis 3, an autosomal recessive liver disorder that progresses to cirrhosis and liver failure before adulthood. Two alternatively spliced human isoforms have been described. |
UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Transporter, ABC family; Hydrolase; EC 3.6.3.44; Membrane protein, integral; Transporter Chromosomal Location of Human Ortholog: 7q21.1 Cellular Component: intercellular canaliculus; membrane; integral to plasma membrane; integral to membrane; plasma membrane Molecular Function:ATPase activity, coupled to transmembrane movement of substances; xenobiotic-transporting ATPase activity; ATP binding Biological Process: response to drug; transport; multidrug transport; xenobiotic transport; lipid metabolic process; cellular lipid metabolic process; transmembrane transport Disease: Gallbladder Disease 1; Cholestasis, Progressive Familial Intrahepatic, 3; Cholestasis, Intrahepatic, Of Pregnancy 3 |
NCBI Summary: | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq, Jul 2008] |
UniProt Code: | P21439 |
NCBI GenInfo Identifier: | 126302568 |
NCBI Gene ID: | 5244 |
NCBI Accession: | P21439.2 |
UniProt Secondary Accession: | P21439,Q14813, A0A2V7, A4D1D3, A4D1D4, A4D1D5, D6W5P3 D6W5P4, |
UniProt Related Accession: | P21439 |
Molecular Weight: | |
NCBI Full Name: | Multidrug resistance protein 3 |
NCBI Synonym Full Names: | ATP-binding cassette, sub-family B (MDR/TAP), member 4 |
NCBI Official Symbol: | ABCB4 |
NCBI Official Synonym Symbols: | GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3 |
NCBI Protein Information: | multidrug resistance protein 3; P-glycoprotein 3; multiple drug resistance 3; ATP-binding cassette sub-family B member 4; P glycoprotein 3/multiple drug resistance 3; P-glycoprotein-3/multiple drug resistance-3 |
UniProt Protein Name: | Multidrug resistance protein 3 |
UniProt Synonym Protein Names: | ATP-binding cassette sub-family B member 4; P-glycoprotein 3 |
Protein Family: | ABC transporter B family |
UniProt Gene Name: | ABCB4 |
UniProt Entry Name: | MDR3_HUMAN |
Antibodies | ELISA Kits |
Anti-ABCB4 Antibody (CAB9835) | Human ABCB4 / MDR3 ELISA Kit |
Human Multidrug resistance protein 3 (ABCB4) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |