AARS Antibody (PACO56712)
- SKU:
- PACO56712
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | AARS Antibody (PACO56712) |
抗体コード: | PACO56712 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:20-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Alanine--tRNA ligase, cytoplasmic protein (164-321AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO56712 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of Hela cells with PACO56712 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO56712 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. |
シノニム: | Alanine--tRNA ligase, cytoplasmic (EC 6.1.1.7) (Alanyl-tRNA synthetase) (AlaRS) (Renal carcinoma antigen NY-REN-42), AARS |
UniProt Protein Function: | AARS: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. Defects in AARS are the cause of Charcot-Marie-Tooth disease type 2N (CMT2N). It is an axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies(designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. Belongs to the class-II aminoacyl-tRNA synthetase family. |
UniProt Protein Details: | Protein type:Aminoacyl-tRNA synthetase; EC 6.1.1.7; Ligase; RNA-binding; Translation; Translation regulation Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: cytoplasm; cytosol; membrane; mitochondrion Molecular Function:alanine-tRNA ligase activity; amino acid binding; tRNA binding Biological Process: alanyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; tRNA modification; tRNA processing Disease: Charcot-marie-tooth Disease, Axonal, Type 2n; Epileptic Encephalopathy, Early Infantile, 29 |
NCBI Summary: | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008] |
UniProt Code: | P49588 |
NCBI GenInfo Identifier: | 115502460 |
NCBI Gene ID: | 16 |
NCBI Accession: | P49588.2 |
UniProt Secondary Accession: | P49588,Q53GV7, Q96FA0, A6NF14, B4DR45, |
UniProt Related Accession: | P49588 |
Molecular Weight: | 107kDa |
NCBI Full Name: | Alanine--tRNA ligase, cytoplasmic |
NCBI Synonym Full Names: | alanyl-tRNA synthetase |
NCBI Official Symbol: | AARS |
NCBI Official Synonym Symbols: | CMT2N; EIEE29 |
NCBI Protein Information: | alanine--tRNA ligase, cytoplasmic |
UniProt Protein Name: | Alanine--tRNA ligase, cytoplasmic |
UniProt Synonym Protein Names: | Alanyl-tRNA synthetase |
UniProt Gene Name: | AARS |
Antibodies |
Anti-AARS Antibody (CAB15017) |
Anti-AARS Antibody (CAB7864) |
Anti-DiMethyl-AARS-K963 Antibody (CAB18162) |
AARS Antibody (PACO07513) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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